The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 05 ng/mL; progesterone: 2. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. Each SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. Microplate Spectrophotometer Operator’s Manual. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. The assays contained 0. Following incubation for 30 min at room temperature, the reaction was quenched by addition of 100 μl stop solution (2 N H 2 SO 4) and absorbance was read using microplate reader (SpectraMax 340; Molecular Devices Co. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. Subsequently, the mixture was heated at 100 °C for 10. Each experiment was performed in duplicate. 0 0 The indicated microplates were scanned form 200 nm to 800 nm. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Integration delay. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 570nm on the indicated days (Cell Proliferation Kit I; Roche). It includes both a lamp and a new lamp holder. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). VersaMax reads 96-well and strip-well plates. Awareness Technology, Inc. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. 315 can't find zero order. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). SpectraMax Multi-Mode Microplate Readers. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. PerkinElmer. Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. , Oxon, UK). Quick View. 94 g calcium chloride dihydrate (both. Molecular Devices Corporation 1311. , San Jose, CA) using Softmax Pro 7 software. 97), and each plate contained at least six positive and six negative controls. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. 3: The cuvette chamber. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. Molecular Device M5e. SpectraMax Plus 384 measuring instruments pdf manual download. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. One plate carrier adapter is provided with the instrument. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Changes in OD 405 nm were followed on the SpectraMAX 340 plate reader. Warranty. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. The initial rate of hydrolysis was determined using the early linear region of. Novak, Nathan J. The concentration of TNFα in the experimental. 7. Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. Microplate Readers. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. The metals were preincubated with A for 5 min when added before heme. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. mums. D2. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. Analysis of ALA by Ehrlich’s Reagent Assay. Bertin Precellys Evolution Touch Homogenizer. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. The Spectra Catalina 340 Automated Connect 12Volt or 24Volt Catalina 340C produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize. Adhesion molecule expression To determine whether baicalein could modify the oxLDL-induced adhesion molecule expression, HUVECs were grown to confluence, pretreated with baicalein for 2 hrs and stimulated with oxLDL (150 μg/ml) for. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 5. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. 1% N-1-naphtylethylenediamine dihydrochloride, 2. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. Revvity. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. 00 USD. 2. Stat Fax 4700 Microstrip Reader. 5 g/L-EDTA 0. 6) and 0. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. Data were expressed as the mean percent viable cell vs. Ehrlich assay for quantification of 5-ALA A 200 μL 5-ALA sample was mixed with 200 μL sodium acetate (pH 4. Labsystems Luminoskan RT Microplate Reader. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. Assay setup for a 384-well low-volume plate. 2. , Sunnyvale, CA, USA) at a wavelength of 450 nm. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). 5mM), and changes in OD 405 nm. They are for reference only. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. The reaction mixture was incubated for 5 min. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. or microplate reader (e. SpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. Manager: Yinghua Chen, PhD Contact Email: yinghua. In vivo anti-malarial assay in mice. The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. The absorbances were being measured at 515 nm. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. 4. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Acceptor filter: 665/10 nm. 313 reference gain check fail. g. On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Setup recommendations for SpectraMax. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Call 1-800-745-2710. Please refer to photos for further. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. reader (Molecular Devi ces). The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. 6. The plate was covered and incubated for one hour at room temperature. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 0 OD. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . All strains were tested three times and the results averaged. Plates were washed with 1× KP solution and blocked with. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). 8. SpectraMax® Microplate Readers and SoftMax® Pro Software. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. 1. All data were normalized to experimental day 0. Donor: 620 nm. Samples were analyzed in triplicates. The growth. 1x10 5 transduced, GFP + SK-NEP-1. 190–850 nm <± 1. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Customer Transformation Hub. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. 5. 1 mL of culture media with 10% FBS. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. Streamline GLP/GMP compliance. Microplate Readers. Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). Buffer (100 mmol L –1acetate buffer, pH 5. All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Subsequent replacements require only the lamp (N-34-LB) be replaced. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Driessen*,MaaikeA. 7 macrophages were obtained from ATCC (Manassas, VA). ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Near bottom. (7. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). Details. , Fremont, CA, USA). SpectraMax Multi-Mode Microplate Readers. edu Contact Phone: 216-368-8962. ) to the upper chamber, followed by measurement with a spectrophotometer (SpectraMax 340, Molecular Devices LLC, Sunnyvale, CA, USA) at 610 nm of media in the lower chamber after one hour . Call 1-800-745-2710. The slope of the linear portion of the kinetic curve generated from each well was used to. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. using SpectraMax i3 (Molecular devices, San Jose, USA). the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. 0200-0729. 5. SpectraMAX 340 microtiter plate reader. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. using a Molecular Dynamics Model SpectraMAX 340 PC microplate reader. 340/70 nm. Up for your consideration is a Molecular Devices SpectraMax 340 Microplate Spectrophotometer Model# 340 Condition is for parts or repair. Data System. 6. This assay measures the two major sugars in wine, glucose and fructose. Microplate Readers. 340-850 nm: Wavelength Selection: Monochromator, turnable in 1 nm increments:. 4. . Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). edu Contact Phone: 216-368-8962. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. 6. S. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. The microtiter plate was then incubated in the dark for 2 h at 37°C. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. SpectraMAX 340. Molecular Devices Corporation 1311. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. Crowder, Ziqian Zeng, Alissa N. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. The sensitivities of the hormones are Prolactin: 0. See More Microplate Reader / Microtiter Plate Reader. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. The cell survival. The reaction was stopped with 1 M hydrochloric acid after 15 min. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The adapter is. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. 00 USD. Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. 0. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. 30. The chamber contains. 1 0 100 80 60 40 20 0 4. Call 1-800-745-2710. Operators can conveniently select up to 6 wavelengths at a time for. The absorbances were transformed to velocity of substrate consumption (nmol/min). When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. , Sunnyvale, CA). The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Molecular Devices SpectraMAX 340 Microplate Reader. Each experiment was performed in duplicate and repeated at. Call 1-800-745-2710. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Not. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. Absorbance was measured at 490/650 nm by using a SpectraMax 340 microplate spectrophotometer® (Molecular Devices, Sunnyvale, CA, U. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. As a common indicator of equine T cell activation, cytokine induction. ). 0 to 4. N-34-LB. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). The light features a slim profile to prevent. 2. Unit has several areas of damaged plastic. 001/min at 425 nm. 4; 100 m M NaCl, 0. Hydrogen peroxide (H 2 O 2) was used to establish an intracellular oxidative. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. 5 ng/ml; estradiol: 10 pg/ml. For FXII, at various times, 90 μL of reaction was mixed with SBTI (500nM) and S-2302 (0. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Molecular Device SpectraMax M3 Plate Reader - Certified with Warranty. albicans pre. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. SpectraMax 190 reads 96-well and strip-well plates. Service parts for these models are not being produced and are no longer in stock. SpectraMAX 340 plate reader (Molecular Devices). 1% N-1-naphtylethylenediamine dihydrochloride, 2. Manager: Yinghua Chen, PhD Contact Email: yinghua. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. (Shiga, Japan) and kept for 30 min in the dark. 304 signal level saturation. Money Saved: $340. Six wells were assayed for each set of conditions and stadard deviations. Amino Acid Analysis. 1: SpectraMax Plus 384 components. The Catalina. Fully tested with Warranty. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. 5. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. well, and strip-well microplates. 1 mL of acetylacetone. Although all samples initially were analyzed at the same volume, the volume of sample. D. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. edu Contact Phone: 216-368-8962. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. ). For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Binding of capture antibodies to polystyrene beads and biotinylation. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Molecular Devices Spectramax 340PC instructions. Upgradeable modules are also available including western blot, cell imaging, and fast. 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. 306 plate air cal. To. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. A 0. The cells were pretreated, serum starved for 24 h, and then treated with NK-cdM priora SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Details. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. SpectraMax 384 Plus. The LDH level in the lavage fluid was calculated based on the standard curve, nor-malized with protein concentration and expressed as ng/mg protein. Multi-mode Microplate Readers. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer. 06 ng/mL; FSH: 0. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). The reaction mixture was incubated for 5 min. SpectraMax Multi-Mode Microplate Readers. Quick View. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. 3. 3 0. . The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. Western blot. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. 1 mL of acetylacetone. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. Fibrin clots were formed as described above with some modifications 46. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. 5. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Create custom fluorescence assays without having to purchase expensive filter sets. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. SPECTRAmax® 190. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Temperature Range: Ambient + 4 °C up to 45 °C. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. Call 1-800-745-2710. The levels of rheumatoid factor (RF) antibodies were measured at the Clinical Immunology Laboratory of the Sahlgrenska University Hospital or Leiden Clinical Immunology Laboratory. The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. Stat Fax 3200 Microplate Reader. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance platereader (Molecular Devices, Union City, CA, United States). Vessel Sprout Formation Assay. Rate of fibrinolysis. -1 activity was determined MMP using an MMP-1 immunoassay kit (dY901; R&d Systems, Inc. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. 2. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. gp70 blocking. MMP‑1 inhibition assay. Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 3. One plate carrier adapter is provided with the instrument. AoSMCs were seeded into 6-well culture plates. SPECTRAMAX 340. 9000-0756. Plates coated with no antigen, canine CD19, streptavidin alone, and streptavidin with irrelevant human avitag-CD3ε (Acro Biosystems, Newark, DE) were used as negative controls. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. Spectral Range. Briefly, cells were seeded into a. Each experiment was performed in duplicate.